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antibody against s1p2  (Proteintech)


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    Structured Review

    Proteintech antibody against s1p2
    Antibody Against S1p2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+s1pr2/S1PR2+Antibody/pm41701442-39-1-7
    Average 94 stars, based on 77 article reviews
    antibody against s1p2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Overexpression of endothelial S1pr2 promotes blood-brain barrier disruption via JNK/c-Jun/MMP-9 pathway after traumatic brain injury in both in vivo and in vitro models.
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes underwent incubation with primary antibodies targeting Erk: anti-Erk and antiphosphorylated Erk (1:1,000; #4370/#4695, CST), JNK: anti-JNK and anti-phosphorylated JNK (1:1,000; #9251/#9252, CST), p38: anti-p38 and anti-phosphorylated p38 (1:1,000; #9212/#9211, CST), c-Jun: Anti-c-Jun and anti-phosphorylated-c-Jun (1:1,000; #9165/ #2361), anti-MMP-9 (1:1,000; ab76003; Abcam), anti-S1pr2 (1:500, 21180-1-AP, Proteintech), anti-Occludin (1:1,000, ab216327, Abcam); anti-BAX (1:1,000, 50599-2-Ig, Proteintech); anti-Bcl-2 (1:1,000, 68103-1-Ig, Proteintech); anti-ZO-1 (1:500, ab96587, Abcam); anti-Occludin (1:1,000, 27260-1-AP, Proteintech); antiβ-actin (1:3,000, AC026, ABclonal). ..

    Article Title: Overexpression of endothelial S1pr2 promotes blood–brain barrier disruption via JNK/c-Jun/MMP-9 pathway after traumatic brain injury in both in vivo and in vitro models
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes underwent incubation with primary antibodies targeting Erk: anti-Erk and anti-phosphorylated Erk (1:1,000; #4370/#4695, CST), JNK: anti-JNK and anti-phosphorylated JNK (1:1,000; #9251/#9252, CST), p38: anti-p38 and anti-phosphorylated p38 (1:1,000; #9212/#9211, CST), c-Jun: Anti-c-Jun and anti-phosphorylated-c-Jun (1:1,000; #9165/#2361), anti-MMP-9 (1:1,000; ab76003; Abcam), anti-S1pr2 (1:500, 21180-1-AP, Proteintech), anti-Occludin (1:1,000, ab216327, Abcam); anti-BAX (1:1,000, 50599-2-Ig, Proteintech); anti-Bcl-2 (1:1,000, 68103-1-Ig, Proteintech); anti-ZO-1 (1:500, ab96587, Abcam); anti-Occludin (1:1,000, 27260-1-AP, Proteintech); anti-β-actin (1:3,000, AC026, ABclonal). ..

    Incubation:

    Article Title: Overexpression of endothelial S1pr2 promotes blood-brain barrier disruption via JNK/c-Jun/MMP-9 pathway after traumatic brain injury in both in vivo and in vitro models.
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes underwent incubation with primary antibodies targeting Erk: anti-Erk and antiphosphorylated Erk (1:1,000; #4370/#4695, CST), JNK: anti-JNK and anti-phosphorylated JNK (1:1,000; #9251/#9252, CST), p38: anti-p38 and anti-phosphorylated p38 (1:1,000; #9212/#9211, CST), c-Jun: Anti-c-Jun and anti-phosphorylated-c-Jun (1:1,000; #9165/ #2361), anti-MMP-9 (1:1,000; ab76003; Abcam), anti-S1pr2 (1:500, 21180-1-AP, Proteintech), anti-Occludin (1:1,000, ab216327, Abcam); anti-BAX (1:1,000, 50599-2-Ig, Proteintech); anti-Bcl-2 (1:1,000, 68103-1-Ig, Proteintech); anti-ZO-1 (1:500, ab96587, Abcam); anti-Occludin (1:1,000, 27260-1-AP, Proteintech); antiβ-actin (1:3,000, AC026, ABclonal). ..

    Article Title: VSV infection and LPS treatment alter serum bile acid profiles, bile acid biosynthesis, and bile acid receptors in mice
    Article Snippet: .. Deparaffinized specimens were incubated with fresh 0.3% hydrogen peroxide and then incubated with anti-FXR (25055-1-AP, 1:300, Proteintech, China), anti-TGR5 (ab72608, 1:300, abcam, UK), anti-S1PR2 (21180-1-AP, 1:300, Proteintech, China), and anti-VDR (67192-1-Ig, 1:300, Proteintech, China) for 1 h at room temperature. ..

    Article Title: Overexpression of endothelial S1pr2 promotes blood–brain barrier disruption via JNK/c-Jun/MMP-9 pathway after traumatic brain injury in both in vivo and in vitro models
    Article Snippet: .. After blocking with 5% non-fat milk, the membranes underwent incubation with primary antibodies targeting Erk: anti-Erk and anti-phosphorylated Erk (1:1,000; #4370/#4695, CST), JNK: anti-JNK and anti-phosphorylated JNK (1:1,000; #9251/#9252, CST), p38: anti-p38 and anti-phosphorylated p38 (1:1,000; #9212/#9211, CST), c-Jun: Anti-c-Jun and anti-phosphorylated-c-Jun (1:1,000; #9165/#2361), anti-MMP-9 (1:1,000; ab76003; Abcam), anti-S1pr2 (1:500, 21180-1-AP, Proteintech), anti-Occludin (1:1,000, ab216327, Abcam); anti-BAX (1:1,000, 50599-2-Ig, Proteintech); anti-Bcl-2 (1:1,000, 68103-1-Ig, Proteintech); anti-ZO-1 (1:500, ab96587, Abcam); anti-Occludin (1:1,000, 27260-1-AP, Proteintech); anti-β-actin (1:3,000, AC026, ABclonal). ..

    Article Title: VSV infection and LPS treatment alter serum bile acid profiles, bile acid biosynthesis, and bile acid receptors in mice.
    Article Snippet: .. Deparaffinized specimens were incubated with fresh 0.3% hydrogen peroxide and then incubated with anti-FXR (25055-1-AP, 1:300, Proteintech, China), anti-TGR5 (ab72608, 1:300, abcam, UK), anti-S1PR2 (21180-1-AP, 1:300, Proteintech, China), and anti-VDR (67192-1-Ig, 1:300, Proteintech, China) for 1 h at room temperature. ..

    other:


    Article Title: Exogenous S1P via S1P receptor 2 induces CTGF expression through Src-RhoA-ROCK-YAP pathway in hepatic stellate cells.
    Article Snippet: 1 Department of Health Science and Technology, SAIHST, Sungkyunkwan University, 81 Irwon-Ro, Gangnam-Gu, Seoul 06351, South Korea 2 Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 IrwonRo, Gangnam-Gu, Seoul 06351, South Korea 3 Samsung Medical Center, 81 IrwonRo, Gangnam-Gu, Seoul 06351, South Korea Abstract Background Hepatic fibrosis, a prevalent chronic liver condition, involves excessive extracellular matrix production associated with aberrant wound healing.. Hepatic stellate cells (HSCs) play a pivotal role in liver fibrosis, activated by inflammatory factors such as sphingosine 1-phosphate (S1P).. Despite S1P’s involvement in fibrosis, its specific role and downstream pathway in HSCs remain controversial.



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    GCDCA induces M2-type polarization in macrophages through activation of <t>S1PR2</t> receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.
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    GCDCA induces M2-type polarization in macrophages through activation of <t>S1PR2</t> receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.
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    GCDCA induces M2-type polarization in macrophages through activation of <t>S1PR2</t> receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.
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    ABclonal Biotechnology anti rabbit s1pr2 antibody
    GCDCA induces M2-type polarization in macrophages through activation of <t>S1PR2</t> receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.
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    Image Search Results


    GCDCA induces M2-type polarization in macrophages through activation of S1PR2 receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Journal: Frontiers in Immunology

    Article Title: GCDCA promotes hepatocellular carcinoma progression through S1PR2/PI3K/AKT-mediated polarization of M2-type macrophages

    doi: 10.3389/fimmu.2026.1640450

    Figure Lengend Snippet: GCDCA induces M2-type polarization in macrophages through activation of S1PR2 receptors. (A) Single-cell sequencing to analyze bile acid receptor expression in M2 macrophages. (B) Activation of the S1PR2 receptor by GCDCA was detected using the Tango GPCR assay (n = 3 in each group). (C, D) TCGA database analysis of S1PR2 correlation with KRT19 , EPCAM , SOX9 , CD68 , MRC1 , CD163 . (E) Representative immunohistochemical staining of CD68, CD163, and S1PR2-positive cells in the livers of different groups of rats, scale bar = 100 μm (n = 3 in each group). (F) Representative immunofluorescence staining of S1PR2 (red) co-localized with CD163- (green) and CD206-(green) positive cells in the livers of different groups of rats, scale bar = 100 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Article Snippet: The following antibodies were used for immunohistochemistry: EPCAM (1:100, ab71916; Abcam), KRT19 (1:4000, 10712-1-AP; Proteintech), SOX9 (1:2000, ab185230; Abcam), CD163 (1:4000, ab213612; Abcam), S1PR2 (1:100, sc-365963; Santa Cruz Biotechnology), and CD68 (1:3000, ab31630; Abcam).

    Techniques: Activation Assay, Single Cell, Sequencing, Expressing, Immunohistochemical staining, Staining, Immunofluorescence

    Pharmacological inhibition of S1PR2 by JTE-013 reverses GCDCA-induced stemness. (A) Flow chart of animal experiments. (B) Kaplan–Meier plots of DEN-induced primary hepatocellular carcinoma in rats after administration of GCDCA and the receptor inhibitor JTE-013 ( n = 5 in each group). (C) Representative images of liver tumors in different groups of rats (scale bar = 1 cm) and HE-stained images (scale bar = 200 μm). (D, E) Analysis of maximum diameter and number of tumors (n = 4 in each group). (F–H) Serum AST and ALT activities and TBA levels in different groups of rats (n = 4 in each group). (I) Representative immunohistochemical staining of KRT19, EPCAM, and SOX9-positive cells in the livers of different groups of rats, scale bar = 200 μm (n = 3 in each group). (J) Representative immunofluorescence staining of KRT19, EPCAM, and SOX9 co-localized positive cells in the livers of different groups of rats, scale bar = 50 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Journal: Frontiers in Immunology

    Article Title: GCDCA promotes hepatocellular carcinoma progression through S1PR2/PI3K/AKT-mediated polarization of M2-type macrophages

    doi: 10.3389/fimmu.2026.1640450

    Figure Lengend Snippet: Pharmacological inhibition of S1PR2 by JTE-013 reverses GCDCA-induced stemness. (A) Flow chart of animal experiments. (B) Kaplan–Meier plots of DEN-induced primary hepatocellular carcinoma in rats after administration of GCDCA and the receptor inhibitor JTE-013 ( n = 5 in each group). (C) Representative images of liver tumors in different groups of rats (scale bar = 1 cm) and HE-stained images (scale bar = 200 μm). (D, E) Analysis of maximum diameter and number of tumors (n = 4 in each group). (F–H) Serum AST and ALT activities and TBA levels in different groups of rats (n = 4 in each group). (I) Representative immunohistochemical staining of KRT19, EPCAM, and SOX9-positive cells in the livers of different groups of rats, scale bar = 200 μm (n = 3 in each group). (J) Representative immunofluorescence staining of KRT19, EPCAM, and SOX9 co-localized positive cells in the livers of different groups of rats, scale bar = 50 μm. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Article Snippet: The following antibodies were used for immunohistochemistry: EPCAM (1:100, ab71916; Abcam), KRT19 (1:4000, 10712-1-AP; Proteintech), SOX9 (1:2000, ab185230; Abcam), CD163 (1:4000, ab213612; Abcam), S1PR2 (1:100, sc-365963; Santa Cruz Biotechnology), and CD68 (1:3000, ab31630; Abcam).

    Techniques: Inhibition, Staining, Immunohistochemical staining, Immunofluorescence

    Inhibition of S1PR2 receptor reverses GCDCA-induced polarization of M2 macrophages. (A) Expression of M2 macrophage marker genes Mrc1 , Cd163 , and Arg1 in NR8383 cells after inhibition of the S1PR2 receptor (n = 3 in each group). (B) Expression of CD206, CD163, and ARG1 proteins in NR8383 cells after inhibition of the S1PR2 receptor. (C) Expression of M2 macrophage marker genes MRC1 , ARG1 , and TGF-β in THP-1 cells after inhibition of the S1PR2 receptor (n = 3 in each group). (D) Expression of CD206, CD163, and ARG1 proteins in THP-1 cells after inhibition of the S1PR2 receptor. (E) Expression of stemness genes Krt19 , Epcam , and Sox9 in RH35 cells after co-culture with GCDCA-induced NR8383 cells pretreated with JTE-013 (n = 3 in each group). (F) Expression of KRT19, EPCAM, and SOX9 proteins in RH35 cells after co-culture with GCDCA-induced NR8383 cells pretreated with JTE-013. (G) Expression of stemness genes KRT19 , EPCAM , and SOX9 in Huh7 cells after co-culture with GCDCA-induced THP-1 cells pretreated with JTE-013 (n = 3 in each group). (H) Expression of KRT19, EPCAM, and SOX9 proteins in Huh7 cells after co-culture with GCDCA-induced THP-1 cells pretreated with JTE-013. (I-J) Representative immunofluorescence staining of M2 macrophage markers CD206 (white) and CD163 (white) co-localized with KRT19- (red) and EPCAM- (green) positive cells in the livers of different groups of rats, scale bar = 100 μm. GAPDH is used as an internal reference. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Journal: Frontiers in Immunology

    Article Title: GCDCA promotes hepatocellular carcinoma progression through S1PR2/PI3K/AKT-mediated polarization of M2-type macrophages

    doi: 10.3389/fimmu.2026.1640450

    Figure Lengend Snippet: Inhibition of S1PR2 receptor reverses GCDCA-induced polarization of M2 macrophages. (A) Expression of M2 macrophage marker genes Mrc1 , Cd163 , and Arg1 in NR8383 cells after inhibition of the S1PR2 receptor (n = 3 in each group). (B) Expression of CD206, CD163, and ARG1 proteins in NR8383 cells after inhibition of the S1PR2 receptor. (C) Expression of M2 macrophage marker genes MRC1 , ARG1 , and TGF-β in THP-1 cells after inhibition of the S1PR2 receptor (n = 3 in each group). (D) Expression of CD206, CD163, and ARG1 proteins in THP-1 cells after inhibition of the S1PR2 receptor. (E) Expression of stemness genes Krt19 , Epcam , and Sox9 in RH35 cells after co-culture with GCDCA-induced NR8383 cells pretreated with JTE-013 (n = 3 in each group). (F) Expression of KRT19, EPCAM, and SOX9 proteins in RH35 cells after co-culture with GCDCA-induced NR8383 cells pretreated with JTE-013. (G) Expression of stemness genes KRT19 , EPCAM , and SOX9 in Huh7 cells after co-culture with GCDCA-induced THP-1 cells pretreated with JTE-013 (n = 3 in each group). (H) Expression of KRT19, EPCAM, and SOX9 proteins in Huh7 cells after co-culture with GCDCA-induced THP-1 cells pretreated with JTE-013. (I-J) Representative immunofluorescence staining of M2 macrophage markers CD206 (white) and CD163 (white) co-localized with KRT19- (red) and EPCAM- (green) positive cells in the livers of different groups of rats, scale bar = 100 μm. GAPDH is used as an internal reference. * p < 0.05, ** p < 0.01, and *** p < 0.001, n.s., not significant.

    Article Snippet: The following antibodies were used for immunohistochemistry: EPCAM (1:100, ab71916; Abcam), KRT19 (1:4000, 10712-1-AP; Proteintech), SOX9 (1:2000, ab185230; Abcam), CD163 (1:4000, ab213612; Abcam), S1PR2 (1:100, sc-365963; Santa Cruz Biotechnology), and CD68 (1:3000, ab31630; Abcam).

    Techniques: Inhibition, Expressing, Marker, Co-Culture Assay, Immunofluorescence, Staining

    GCDCA induces macrophage polarization toward M2 via the S1PR2/PI3K/AKT signaling pathway. (A) Volcano plot of differentially expressed genes (DEGs) between GCDCA-treated and control groups (n = 3 in each group). (B) Histogram of KEGG enrichment results of DEGs between GCDCA-treated and control groups. (C) Bubble plots of GO enrichment results of DEGs between GCDCA-treated and control groups. (D) Western blotting for p-AKT, AKT, p-PI3K, and PI3K protein expression in GCDCA-treated NR8383 cells. (E) Western blotting was used to detect the expression of p-AKT, AKT, p-PI3K, PI3K, CD206, and ARG1 proteins after pretreatment with LY294002 inhibitor.

    Journal: Frontiers in Immunology

    Article Title: GCDCA promotes hepatocellular carcinoma progression through S1PR2/PI3K/AKT-mediated polarization of M2-type macrophages

    doi: 10.3389/fimmu.2026.1640450

    Figure Lengend Snippet: GCDCA induces macrophage polarization toward M2 via the S1PR2/PI3K/AKT signaling pathway. (A) Volcano plot of differentially expressed genes (DEGs) between GCDCA-treated and control groups (n = 3 in each group). (B) Histogram of KEGG enrichment results of DEGs between GCDCA-treated and control groups. (C) Bubble plots of GO enrichment results of DEGs between GCDCA-treated and control groups. (D) Western blotting for p-AKT, AKT, p-PI3K, and PI3K protein expression in GCDCA-treated NR8383 cells. (E) Western blotting was used to detect the expression of p-AKT, AKT, p-PI3K, PI3K, CD206, and ARG1 proteins after pretreatment with LY294002 inhibitor.

    Article Snippet: The following antibodies were used for immunohistochemistry: EPCAM (1:100, ab71916; Abcam), KRT19 (1:4000, 10712-1-AP; Proteintech), SOX9 (1:2000, ab185230; Abcam), CD163 (1:4000, ab213612; Abcam), S1PR2 (1:100, sc-365963; Santa Cruz Biotechnology), and CD68 (1:3000, ab31630; Abcam).

    Techniques: Control, Western Blot, Expressing